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Repeat Breeding & Infertility in Cows & Buffaloes
Disease guide CowBuffalo

Repeat Breeding & Infertility in Cows & Buffaloes

Repeat breeding is when a healthy-looking cow or buffalo keeps coming back into heat after mating or insemination and does not conceive. Hormone, pregnancy, uterine swab, venereal disease and mineral tests help a vet look for the cause.

Signs owners may notice

  • Coming back into heat after each mating or insemination
  • Normal, regular heats but no pregnancy
  • Weak or missed heat signs (silent heat), common in buffaloes
  • No heat for a long time after calving
  • Cloudy or pus-like discharge from the vulva, which may point to uterine infection
  • Irregular heats that come too soon or too late
  • Returning to heat after a pregnancy was thought to be confirmed

Signs alone cannot name a disease. If your animal looks unwell, see a veterinarian promptly.

How lab tests help

Progesterone in blood or milk shows whether the ovaries are cycling and helps check heat timing, and PAG helps confirm pregnancy. A uterine swab culture can find bacteria in the womb, and vaginal cytology can show inflammation. Venereal disease tests look for germs spread at mating, and mineral profiles check nutrition. A registered veterinarian reads the results with the breeding history.

What is repeat breeding?

A repeat breeder is a cow or buffalo that keeps coming back into heat after being mated or inseminated, even though she seems healthy and her heats look normal. Infertility is the wider problem of an animal that does not get pregnant, or does not come into heat at all. Both cost dairy farmers milk, calves and time.

There are many possible causes. Heat may be missed, or insemination may be done at the wrong time. The embryo may die very early. A hidden infection of the uterus (womb) is common after a difficult calving or a retained afterbirth. Hormone imbalance, poor body condition, heat stress and shortages of minerals such as phosphorus, copper or zinc can all play a part. Some germs spread at natural mating, and bulls can carry them without signs. Buffaloes often show weak or silent heat, so heat is easy to miss.

Signs to watch for

Common signs include:

  • Coming back into heat after each insemination
  • Regular heats but no pregnancy
  • Weak or silent heat, common in buffaloes
  • No heat for a long time after calving
  • Cloudy or pus-like discharge from the vulva
  • Irregular heats, or returning to heat late

Not every animal shows every sign. A late return to heat may mean an early pregnancy was lost.

Which lab tests help

Progesterone, Serum shows whether the ovaries are working through the heat cycle and helps your vet check heat timing. Pregnancy-Associated Glycoprotein (PAG) ELISA helps confirm pregnancy. PAG can stay in the blood for a while after calving or a lost pregnancy, so your vet reads it with the breeding dates.

A Uterine Discharge Swab Culture & Sensitivity grows bacteria from the uterus and shows which medicines may work, which helps your vet choose. Vaginal Cytology looks at cells under a microscope and can show inflammation. Where a bull is used for natural mating, Trichomonas foetus (Bull) - Culture & RT-PCR and Campylobacter fetus (Bull) - Culture & RT-PCR look for two germs spread at mating, and Bull Semen Evaluation checks the bull's semen. The Mineral Profile (Calcium, Phosphorus & Magnesium) and Trace Mineral Profile for Cattle & Buffalo (Copper, Zinc & Selenium) check minerals linked with fertility.

A registered veterinarian interprets every result together with the animal's signs and breeding history. The lab confirms the method, sample needs and reporting time when you book.

When to see your vet

See your vet promptly if a cow or buffalo has returned to heat several times, has no heat long after calving, or has an abnormal discharge. Keep a record of heat, insemination and calving dates, because it helps your vet a lot. A cow with a foul discharge, fever or poor appetite after calving needs a vet the same day. Ask your vet about prevention and herd fertility plans.

Testing in Bhopal

Pashupatinath Vet Lab at C-200, C Sector, Shahpura, Bhopal provides free sample collection anywhere in Bhopal. Farmers in other districts can send samples through their local vet, by courier or by bus parcel. Phone or WhatsApp +91 88175 01118 to arrange it. Reports come on WhatsApp, by e-mail and in My Reports on the website.

Lab tests that may help

Packages

Confirm pregnancy and screen for a common cause of abortion.

  • Pregnancy Diagnosis
  • Progesterone
  • + 1 more tests

Call to confirm

Common questions

Why does my cow keep coming into heat after insemination?
A cow that keeps coming into heat after insemination may have a hidden uterine infection, wrong insemination timing, early embryo loss, a hormone imbalance or a mineral shortage. Your vet may examine her and use progesterone, uterine swab and mineral tests to look for the cause. Keep a record of heat and insemination dates.
How is progesterone testing used in cows and buffaloes?
Progesterone testing shows whether a cow or buffalo has an active yellow body on the ovary, which makes this hormone. Low progesterone fits with heat, while higher progesterone suggests the cycle has moved on or she may be pregnant. Your vet reads the result with the heat and breeding dates.
Can a bull cause repeat breeding in a herd?
Yes, a bull can cause repeat breeding if his semen quality is poor or if he carries a germ spread at mating, such as Tritrichomonas foetus or Campylobacter fetus, often without looking ill. Bull semen evaluation and these germ tests help your vet check. A registered veterinarian interprets the results.
What is silent heat in buffaloes?
Silent heat means a buffalo is in heat but shows weak or no outward signs, so the heat is easily missed. It is common in buffaloes, especially in hot weather. Progesterone tests in blood or milk can help your vet work out where she is in her cycle.

This guide is general information for animal owners. It does not diagnose or treat any animal. A registered veterinarian should examine the animal and read all test results together with its signs and history.

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