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Leptospirosis in Dogs, Cattle & Buffalo
Disease guide DogCowBuffalo

Leptospirosis in Dogs, Cattle & Buffalo

Leptospirosis is a bacterial infection spread mainly through the urine of infected animals and dirty water, and it can pass to people. PCR, MAT, ELISA and kidney and liver tests can help your vet detect it in dogs, cows and buffaloes.

Signs owners may notice

  • Fever, low energy and poor appetite
  • Vomiting, belly pain or stiff, painful muscles in dogs
  • Drinking more, or passing more or less urine than usual
  • Yellow eyes, gums or skin (jaundice)
  • Small red spots on the gums, or red or dark urine
  • Abortion, stillbirth or weak calves in cows and buffaloes
  • A sudden fall in milk, with thick, yellowish or blood-stained milk

Signs alone cannot name a disease. If your animal looks unwell, see a veterinarian promptly.

How lab tests help

PCR can find Leptospira genetic material in blood early in the illness and in urine later. MAT and ELISA look for antibodies, and a second sample a few weeks later may be needed to show a rising level. Kidney, liver and urine tests show organ damage. Vaccination can affect antibody results, so a registered veterinarian reads all results with the animal's signs and history.

What is leptospirosis?

Leptospirosis is an infection caused by Leptospira bacteria. Infected animals, especially rats, pass the bacteria in their urine. The bacteria survive for a long time in wet soil and water, so cases often rise in the rainy season. Dogs, cows and buffaloes catch it through cuts in the skin or through the mouth, nose and eyes.

In dogs, leptospirosis can damage the kidneys and liver, and some dogs bleed or struggle to breathe. In cows and buffaloes it is often mild or hidden, but it can cause abortion, weak calves, poor fertility and a sudden drop in milk. Young calves can become seriously ill.

Leptospirosis can pass to people. Wear gloves when handling urine, sick animals, aborted calves or samples, wash your hands well, and see a doctor if you feel unwell.

Signs to watch for

Common signs include:

  • Fever, tiredness and poor appetite
  • Vomiting, belly pain or stiff muscles in dogs
  • Drinking more, or passing more or less urine
  • Yellow eyes, gums or skin
  • Abortion, stillbirth or weak calves in cows and buffaloes
  • A sudden fall in milk, with thick or blood-stained milk

Some animals look healthy but still pass the bacteria in urine.

Which lab tests help

The Leptospira RT-PCR looks for the bacteria's genetic material, in blood early in the illness and in urine later. Your vet may choose to collect samples before starting antibiotics, because these make the bacteria harder to find.

The Leptospira MAT (Microscopic Agglutination Test) (MAT) is the reference antibody test. Antibodies take some days to appear, so an early sample can be negative, and your vet may ask for a second sample a few weeks later to look for a rising level. The Leptospira IgM / IgG ELISA is another antibody test, and the Canine Leptospira (Rapid Test) is a quick kit for dogs. Vaccination can also raise antibodies, so tell your vet about past vaccines.

The Kidney Function Test (KFT), Liver Function Test (LFT) and Urine Routine show how the kidneys and liver are coping. For abortions in cows and buffaloes, the Abortion Investigation Panel for Cattle & Buffalo looks at several common causes together; the exact list is confirmed when you book, and many abortions stay unexplained.

A registered veterinarian reads each result with the animal's signs and history.

When to see your vet

See your vet promptly if a dog has fever, vomiting, yellow gums or changes in urine, especially after contact with dirty water or rats. A dog that stops passing urine, bleeds or struggles to breathe needs a vet the same day. Farmers who see abortions, weak calves or a sudden fall in milk in several animals should tell their veterinarian or the animal husbandry department. Ask your vet about vaccination and prevention.

Testing in Bhopal

Pashupatinath Vet Lab is at C-200, C Sector, Shahpura, Bhopal, and sample collection is free anywhere in Bhopal. Farmers and dog owners in other districts can send samples by courier, by bus parcel or through their local vet; call or WhatsApp +91 88175 01118 first. The lab confirms the method, sample needs and reporting time when you book. Reports come on WhatsApp, by e-mail and in My Reports on the website.

Lab tests that may help

Common questions

How is leptospirosis tested in dogs?
Leptospirosis in dogs is usually tested with a mix of tests. PCR on blood or urine looks for the bacteria, while MAT, ELISA or a rapid kit look for antibodies. Kidney, liver and urine tests show organ damage. A registered veterinarian reads the results with the dog's signs and vaccine history.
Can leptospirosis spread from dogs or cattle to humans?
Yes, leptospirosis can pass to people, mainly through contact with the urine of infected animals or with contaminated water and soil. Wear gloves when handling urine, sick animals or aborted calves, wash your hands well, and see a doctor if you develop a fever or feel unwell.
Why does my vet want a second leptospirosis blood test?
A second test helps because antibodies take time to rise. An early MAT can be negative or show a low level that is hard to read, especially in vaccinated animals. A second sample a few weeks later can show whether the level is rising, which points to a recent infection. Your vet decides the timing.
Does leptospirosis cause abortion in cows and buffaloes?
Yes, leptospirosis is one possible cause of abortion, stillbirth and weak calves in cows and buffaloes, and it can also cause a sudden fall in milk. Many other infections cause abortion too, so your vet may test for several causes. Tell your veterinarian or the animal husbandry department if several animals are affected.

This guide is general information for animal owners. It does not diagnose or treat any animal. A registered veterinarian should examine the animal and read all test results together with its signs and history.

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