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HS RT-PCR

Haemorrhagic Septicaemia (Pasteurella multocida) RT-PCR

HS RT-PCR is a test that looks for the DNA of Pasteurella multocida, the germ behind haemorrhagic septicaemia (HS, called galghotu in Hindi), a fast and often fatal disease of cows and buffaloes. It can help a vet confirm HS from blood, nasal swabs or tissue.

Sample
Whole blood, nasal swab or tissue
Quantity
As advised
Report
Confirmed when you book
Collection
Free in Bhopal

What this test checks

  • DNA of Pasteurella multocida, the germ that causes HS, in blood, swab or tissue samples
  • The germ in blood, once it has spread there in the later stage of the illness
  • The germ in tissue taken by a vet from an animal that died suddenly
  • Carrier animals that hold the germ in the nose or throat, using nasal swabs

Benefits of this test

  • Can help confirm HS as the cause of sudden illness or death in a herd.
  • Gives your vet clearer information to plan care for the other animals.
  • Can be done on blood, nasal swabs or tissue, depending on the case.
  • A negative result helps your vet decide whether to look for other causes of sudden death.
  • Nasal swabs can help your vet look for carrier animals.

When your vet may advise it

  • A buffalo or cow with sudden high fever, drooling and a swollen throat or neck.
  • Noisy or difficult breathing with fever, especially in the rainy season.
  • Sudden death of one or more animals with no clear cause.
  • Several animals in a herd or village falling ill quickly at the same time.
  • After a case, when your vet wants to check other animals in the herd for the germ.

About this test

Haemorrhagic septicaemia (HS), known in Hindi as galghotu, is a severe bacterial disease of cows and buffaloes. It is caused by certain types of Pasteurella multocida, a germ that some healthy animals carry in the nose and throat. Buffaloes are more likely to get HS than cows, and most outbreaks happen in the rainy season. Signs come on suddenly: high fever, dullness, drooling, a hot, painful swelling of the throat and neck, and noisy, difficult breathing. The disease moves very fast, and some animals are simply found dead.

PCR (polymerase chain reaction) is a lab method that finds a germ's genetic material, called DNA, by copying it many times. This test looks for Pasteurella multocida DNA. In a sick animal, the vet may take blood, as the germ spreads into the blood when the disease advances. Nasal swabs can help look for animals that carry the germ. From an animal that has died, a veterinarian takes tissue samples. Never cut open a dead animal yourself, because some causes of sudden death, such as anthrax, can infect people. Wear gloves when handling sick animals, wash your hands afterwards and see a doctor if you feel unwell.

Because HS moves so fast, call a vet at once if you see the signs, and never let treatment wait for a lab result. The test is most useful to confirm why animals fell ill or died, so your vet can plan care for the rest of the herd. If several animals fall ill or die suddenly, tell your veterinarian or the animal husbandry department. A registered veterinarian reads the result together with the signs, the herd's history, the season and any post-mortem findings.

How to prepare

A veterinarian takes the samples, if possible before antibiotics are given, but a sick animal's treatment must never be delayed for the test, and only a vet should take samples from a dead animal. The lab confirms the method, sample needs and reporting time when you book.

Reading the result

A registered veterinarian reads the result together with the animal's signs and history, the season and any post-mortem findings. Some types of Pasteurella multocida live harmlessly in the nose or cause pneumonia rather than HS, so a positive nasal swab does not confirm HS on its own. A negative result does not always rule HS out, for example if the sample was taken early or after treatment.

Questions about HS RT-PCR

What is galghotu (HS) in buffaloes and cows?
Galghotu, or haemorrhagic septicaemia (HS), is a sudden and often fatal bacterial disease of buffaloes and cows caused by Pasteurella multocida. It brings high fever, a swollen throat and hard breathing, most often in the rainy season. Call a vet at once if you see these signs.
Should I wait for the HS PCR result before calling the vet?
No. HS can kill an animal very quickly, so call a veterinarian as soon as an animal shows signs. The test helps confirm the cause and guide care for the rest of the herd, but treatment decisions should never wait for it.
Which sample is used for the HS PCR test?
A vet may use blood from a sick animal, a nasal swab to look for carriers, or tissue from an animal that has died. Only a veterinarian should take samples from a dead animal. The lab confirms the sample needs when you book.
Can a healthy buffalo carry the HS germ?
Yes. Some healthy cows and buffaloes carry Pasteurella multocida in the nose and throat. Stress, such as hard work, transport or hot and humid weather, can help bring on the disease. A positive swab from a healthy animal is read by your vet with the herd history.

Before you book this test

Why this notice: so you know, before you book, how a test is done, what its limits are and who is responsible.

  • Where it is tested. Some tests are done wholly or partly by an independent partner laboratory. The lab tells you before booking if this test is sent out, and the report names the laboratory that did it.
  • What a result can and cannot tell you. No test is right every time. A normal result does not rule out every disease, and an abnormal result does not prove one. A registered veterinarian should read the report with the animal’s signs and history before any treatment is started, changed or stopped.
  • The sample. A sample that is clotted, too small, wrongly labelled, leaking or too old may be rejected or reported with a note, and the lab may ask for a fresh one.
  • Reporting time. Confirmed when you book. Times are estimates, not guarantees, and the lab confirms the time when you book.
  • Emergencies. A booking or a pending report must not delay urgent care. If an animal has collapsed, struggles to breathe, has fits or bleeds heavily, call a veterinarian at once.

Please read our Terms of Service, Medical Disclaimer, Privacy Policy and Refund and Cancellation Policy. Pashupatinath Vet Lab is operated by Duaji Healthcare and Tech Private Limited. This page is general information and does not replace a veterinarian’s advice.

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