RT-PCR / Molecular Tests Dog Cow Buffalo

Neospora caninum RT-PCR

Neospora caninum RT-PCR looks for the DNA of the Neospora parasite in blood or tissue. It is commonly used to look into abortion in cows and buffaloes, and nerve and muscle disease in puppies.

Sample: EDTA blood or tissue (for example an aborted calf)
Quantity: As advised
Report: Confirmed when you book
Collection: Free in Bhopal

What this test checks

Neospora caninum DNA in EDTA blood or in tissue, such as from an aborted calf
Whether the parasite is present in an abortion case or in a puppy with nerve or muscle signs

When your vet may advise it

A cow or buffalo aborts, especially when more than one abortion happens in the herd
Your vet is looking into an abortion and has tissue from the aborted calf
A puppy develops stiff, weak or paralysed hind legs
An adult dog has unexplained muscle weakness or nerve signs

About this test

Neospora caninum is a parasite. Dogs are its main host and pass its egg-like forms in their faeces. Cows and buffaloes can be infected by eating feed or drinking water soiled by dog faeces, and an infected mother can pass it to the calf in the womb. In cattle and buffaloes it is a known cause of abortion. In puppies it can cause stiff, weak hind legs that slowly get worse. PCR detects parasite DNA in blood or tissue, such as the brain of an aborted calf. A negative result does not rule infection out. A registered veterinarian reads it with antibody tests, the history and other findings.

How to prepare

Your vet takes blood or collects tissue from an aborted calf. Keep the aborted calf and afterbirth cool, covered and away from dogs until the vet sees them. The lab confirms the method, sample needs and reporting time when you book.

Questions about Neospora caninum RT-PCR

Can dogs spread Neospora to cows and buffaloes?

Yes. Infected dogs can pass the parasite's egg-like forms in their faeces, and cattle and buffaloes can swallow them with feed or water. Dogs can pick up the parasite by eating aborted material or afterbirth. Your vet can advise on keeping dogs away from feed stores and calving areas.

Which sample is used for Neospora PCR after an abortion?

Tissue from the aborted calf, often the brain, is commonly used, and your vet decides what to collect. Wear gloves and wash your hands when handling aborted material, because other causes of abortion, such as brucellosis, can infect people.

Before you book this test

Why this notice: so you know, before you book, how a test is done, what its limits are and who is responsible.

Where it is tested. Some tests are done wholly or partly by an independent partner laboratory. The lab tells you before booking if this test is sent out, and the report names the laboratory that did it.
What a result can and cannot tell you. No test is right every time. A normal result does not rule out every disease, and an abnormal result does not prove one. A registered veterinarian should read the report with the animal’s signs and history before any treatment is started, changed or stopped.
The sample. A sample that is clotted, too small, wrongly labelled, leaking or too old may be rejected or reported with a note, and the lab may ask for a fresh one.
Reporting time. Confirmed when you book. Times are estimates, not guarantees, and the lab confirms the time when you book.
Emergencies. A booking or a pending report must not delay urgent care. If an animal has collapsed, struggles to breathe, has fits or bleeds heavily, call a veterinarian at once.

Please read our Terms of Service, Medical Disclaimer, Privacy Policy and Refund and Cancellation Policy. Pashupatinath Vet Lab is operated by Duaji Healthcare and Tech Private Limited. This page is general information and does not replace a veterinarian’s advice.

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