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Anaplasma marginale RT-PCR

Anaplasma marginale RT-PCR

Anaplasma marginale RT-PCR looks for the DNA of Anaplasma marginale, a tick-borne bacterium that lives in red blood cells, in a blood sample. It can help your vet confirm anaplasmosis, a cause of anaemia in cows and buffaloes.

Sample
Whole blood (EDTA)
Quantity
As advised
Report
Confirmed when you book
Collection
Free in Bhopal

What this test checks

  • Anaplasma marginale DNA in whole blood (EDTA) from a cow or buffalo
  • Low-level infection in carrier animals that a blood smear may not show

When your vet may advise it

  • Fever, pale or yellow eyes and weakness in an adult cow or buffalo
  • Anaemia without red urine in a herd with ticks or biting flies
  • A blood smear is unclear or negative but your vet still suspects anaplasmosis
  • Finding carrier animals in a herd where anaplasmosis keeps returning

About this test

Anaplasmosis is caused by Anaplasma marginale, a bacterium that lives inside red blood cells. It is spread by ticks, biting flies and blood carried on shared needles or tools. The body destroys the infected red cells, which leads to anaemia, meaning too few red blood cells. Signs include fever, pale or yellow eyes, weakness, weight loss and a drop in milk, but usually not red urine. Adult animals are often hit harder than young ones. Animals that recover can stay carriers for life. PCR can find small amounts of its DNA that a smear may miss. A registered veterinarian interprets the result with the signs and other blood tests.

How to prepare

Your vet collects blood into an EDTA tube, using a clean needle for each animal. Tell the vet about any recent medicines. The lab confirms the method, sample needs and reporting time when you book.

Questions about Anaplasma marginale RT-PCR

How is anaplasmosis different from babesiosis in cattle?
Both are tick-borne blood diseases that cause fever and anaemia, but babesiosis often turns the urine red, while anaplasmosis usually does not. They are caused by different germs, so your vet may ask for PCR or blood smears for both when an animal has tick fever signs.
Can an Anaplasma carrier look healthy?
Yes. Cows and buffaloes that recover from anaplasmosis can carry the bacteria for life without looking ill, and they can be a source of infection for others. PCR can help find these carriers when your vet wants to check a herd.

Before you book this test

Why this notice: so you know, before you book, how a test is done, what its limits are and who is responsible.

  • Where it is tested. Some tests are done wholly or partly by an independent partner laboratory. The lab tells you before booking if this test is sent out, and the report names the laboratory that did it.
  • What a result can and cannot tell you. No test is right every time. A normal result does not rule out every disease, and an abnormal result does not prove one. A registered veterinarian should read the report with the animal’s signs and history before any treatment is started, changed or stopped.
  • The sample. A sample that is clotted, too small, wrongly labelled, leaking or too old may be rejected or reported with a note, and the lab may ask for a fresh one.
  • Reporting time. Confirmed when you book. Times are estimates, not guarantees, and the lab confirms the time when you book.
  • Emergencies. A booking or a pending report must not delay urgent care. If an animal has collapsed, struggles to breathe, has fits or bleeds heavily, call a veterinarian at once.

Please read our Terms of Service, Medical Disclaimer, Privacy Policy and Refund and Cancellation Policy. Pashupatinath Vet Lab is operated by Duaji Healthcare and Tech Private Limited. This page is general information and does not replace a veterinarian’s advice.

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Tick Fever RT-PCR in Cattle and Buffalo: Beyond the Smear

A smear shows tick fever parasites you can see under a microscope, while tick fever RT-PCR can also find light infections and carriers that a smear may miss. Together they give a vet a fuller picture.

18 Oct 2026

Anaplasma marginale RT-PCR

Price on request

Ready to get your animal tested?

Book online in two minutes. We collect the sample free of charge anywhere in Bhopal, or you can send it from any district of Madhya Pradesh.

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