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Mycoplasma bovis Milk RT-PCR

Mycoplasma bovis Milk RT-PCR

The Mycoplasma bovis milk RT-PCR looks for the DNA of Mycoplasma bovis, a contagious mastitis germ, in milk from cows and buffaloes. It can find this germ, which routine milk culture often misses.

Sample
Aseptic milk sample
Quantity
As advised
Report
Confirmed when you book
Collection
Free in Bhopal

What this test checks

  • DNA of Mycoplasma bovis in a clean milk sample
  • Whether this hard-to-grow mastitis germ is present in the animals tested

When your vet may advise it

  • Mastitis in several quarters or several animals that does not respond to usual treatment
  • A sudden rise in mastitis cases after new animals join the herd
  • Mastitis in a herd along with pneumonia or swollen joints in calves
  • Routine milk culture shows no growth but mastitis continues

About this test

Mastitis is infection and swelling of the udder. Mycoplasma bovis is a bacterium without a cell wall that spreads from animal to animal at milking, on hands, cloths and machines. It can affect several quarters, cause a sudden drop in milk and respond poorly to usual treatment, while the animal may otherwise seem well. In calves it can also cause pneumonia and swollen joints. Routine milk culture does not give it the special conditions it needs, so PCR is used to detect its DNA. Shedding into milk can come and go, so one negative sample may miss it. A registered veterinarian interprets the result with the herd's mastitis history.

How to prepare

Clean and dry the teat, strip away the first milk, wipe the teat end with spirit and fill a sterile tube without touching its inside, before antibiotics. The lab confirms the method, sample needs and reporting time when you book.

Questions about Mycoplasma bovis Milk RT-PCR

Why does routine milk culture miss Mycoplasma mastitis?
Mycoplasma bovis needs special growth media and conditions that a routine milk culture does not use, so it often shows as no growth. PCR looks for the germ's DNA instead, which is why vets ask for it when mastitis keeps returning in spite of treatment.
Who should test milk for Mycoplasma bovis?
Dairy farmers, cooperatives and vets usually ask for this test when mastitis spreads in a herd or fails to respond to treatment. The result guides herd decisions with your vet. It is for the owner's information and does not replace official food-safety testing.

Before you book this test

Why this notice: so you know, before you book, how a test is done, what its limits are and who is responsible.

  • Where it is tested. Some tests are done wholly or partly by an independent partner laboratory. The lab tells you before booking if this test is sent out, and the report names the laboratory that did it.
  • What a result can and cannot tell you. No test is right every time. A normal result does not rule out every disease, and an abnormal result does not prove one. A registered veterinarian should read the report with the animal’s signs and history before any treatment is started, changed or stopped.
  • The sample. A sample that is clotted, too small, wrongly labelled, leaking or too old may be rejected or reported with a note, and the lab may ask for a fresh one.
  • Reporting time. Confirmed when you book. Times are estimates, not guarantees, and the lab confirms the time when you book.
  • Emergencies. A booking or a pending report must not delay urgent care. If an animal has collapsed, struggles to breathe, has fits or bleeds heavily, call a veterinarian at once.

Please read our Terms of Service, Medical Disclaimer, Privacy Policy and Refund and Cancellation Policy. Pashupatinath Vet Lab is operated by Duaji Healthcare and Tech Private Limited. This page is general information and does not replace a veterinarian’s advice.

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Ready to get your animal tested?

Book online in two minutes. We collect the sample free of charge anywhere in Bhopal, or you can send it from any district of Madhya Pradesh.

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