Milk Progesterone ELISA
A milk progesterone ELISA measures the hormone progesterone in milk to show where a cow or buffalo is in her heat cycle. It can help confirm that an animal is not in heat or may be pregnant, but it cannot prove pregnancy.
- Sample
- Fresh milk
- Quantity
- As advised
- Report
- Confirmed when you book
- Collection
- Free in Bhopal
What this test checks
- Progesterone, a hormone made on the ovary after ovulation, measured in milk
- Low progesterone, which fits with an animal being in heat
- High progesterone, seen between heats and during pregnancy
- Changes in progesterone over repeated samples, when your vet tracks the cycle
Benefits of this test
- Uses a milk sample, so no blood needs to be taken.
- Can help confirm that the animal was in heat at the time of insemination.
- Can give an early sign that an animal is not pregnant, so your vet can plan the next step.
- Helps your vet look into animals with weak or no heat signs, which is common in buffaloes.
- Can be repeated to follow the heat cycle over time.
When your vet may advise it
- On the day of insemination or natural mating, to check the animal was truly in heat.
- After insemination, at a time your vet advises, to look for early signs of non-pregnancy.
- A cow or buffalo that has not shown heat for a long time after calving.
- Animals that keep coming back into heat after several inseminations (repeat breeders).
- Buffaloes with weak or silent heat signs that are hard to spot.
About this test
A milk progesterone ELISA measures the hormone progesterone in a cow's or buffalo's milk. Progesterone is made on the ovary after the animal ovulates (releases an egg). It stays high between heats and during pregnancy, and it drops low when the animal comes into heat. Because the level in milk follows the level in blood, a milk sample can show where she is in her heat (oestrous) cycle without taking blood.
ELISA (enzyme-linked immunosorbent assay) is a lab method that uses antibodies and a colour change to measure a substance. A low progesterone result on the day of insemination supports that the animal was truly in heat. Later, at a time your vet chooses, a low result suggests she is not pregnant, so your vet can plan the next step. A high result means she may be pregnant, but other conditions can also keep progesterone high.
A high result is never proof of pregnancy. Pregnancy should be confirmed by your vet through an examination or another test, such as a pregnancy-associated glycoprotein (PAG) test. Milk progesterone can also help your vet look into animals that do not show heat, which is common in buffaloes because their heat signs are often weak. A registered veterinarian reads the result with the breeding dates and an examination of the animal. This is a breeding test, not a milk-quality or food-safety test, and the result is for the owner's and vet's information. Farmers in Bhopal and nearby districts can book by phone or WhatsApp.
How to prepare
Collect fresh milk into a clean, labelled bottle at the milking your vet advises, write the date of the last heat or insemination on the label, and keep it cool. The lab confirms the method, sample needs and reporting time when you book.
Reading the result
A registered veterinarian reads the result with the breeding dates and an examination of the animal. A low result usually means the animal is not pregnant at that time. A high result does not prove pregnancy, because other conditions, such as an irregular cycle or early loss of a pregnancy, can keep progesterone high. Pregnancy should be confirmed by your vet.
Questions about Milk Progesterone ELISA
Can a milk test tell if my cow is pregnant?
When should milk be sent for a progesterone test after insemination?
Why is milk progesterone useful for buffaloes?
Is milk progesterone the same as a blood progesterone test?
Before you book this test
Why this notice: so you know, before you book, how a test is done, what its limits are and who is responsible.
- Where it is tested. Some tests are done wholly or partly by an independent partner laboratory. The lab tells you before booking if this test is sent out, and the report names the laboratory that did it.
- What a result can and cannot tell you. No test is right every time. A normal result does not rule out every disease, and an abnormal result does not prove one. A registered veterinarian should read the report with the animal’s signs and history before any treatment is started, changed or stopped.
- The sample. A sample that is clotted, too small, wrongly labelled, leaking or too old may be rejected or reported with a note, and the lab may ask for a fresh one.
- Reporting time. Confirmed when you book. Times are estimates, not guarantees, and the lab confirms the time when you book.
- Emergencies. A booking or a pending report must not delay urgent care. If an animal has collapsed, struggles to breathe, has fits or bleeds heavily, call a veterinarian at once.
Please read our Terms of Service, Medical Disclaimer, Privacy Policy and Refund and Cancellation Policy. Pashupatinath Vet Lab is operated by Duaji Healthcare and Tech Private Limited. This page is general information and does not replace a veterinarian’s advice.
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Milk Progesterone ELISA: Bhopal and the big cities of Madhya Pradesh
Free sample collection anywhere in Bhopal. From the other cities, send the sample by courier, bus parcel or through your local veterinarian, and the report comes to you online.
Free collection in these Bhopal areas
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Milk Progesterone and PAG Tests for Early Pregnancy Checks
Two lab tests help check pregnancy in cows and buffaloes: a progesterone test on milk or blood, which follows the hormone of the cycle, and a PAG test, which looks for a protein made by the placenta. Each has limits, so a vet reads them with the service record.
18 Oct 2026