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FMD NSP (3ABC) Antibody ELISA

FMD NSP (3ABC) Antibody ELISA

The FMD NSP (3ABC) antibody ELISA looks for antibodies to non-structural proteins of foot-and-mouth disease virus in cows and buffaloes. These antibodies point to past or current infection rather than vaccination.

Sample
Serum
Quantity
As advised
Report
Confirmed when you book
Collection
Free in Bhopal

What this test checks

  • Antibodies to the 3ABC non-structural protein (NSP) of foot-and-mouth disease virus
  • Signs of past or current FMD infection, even in vaccinated cows and buffaloes
  • A positive, negative or doubtful result, read against the range on the lab report

Benefits of this test

  • Can help tell infected animals from animals that were only vaccinated.
  • Useful for herd surveillance and for checking animals before purchase or movement.
  • Can help find animals that were infected in the past and show no signs now.
  • Supports your vet and the animal husbandry department in FMD investigations.

When your vet may advise it

  • Before buying cows or buffaloes, or moving them to a new herd or area.
  • After FMD cases in the area, when your vet wants to check the herd.
  • Herd surveillance advised by your vet or the animal husbandry department.
  • When your vet wants to know whether vaccinated animals have also been infected.

About this test

Foot-and-mouth disease, or FMD, is a highly contagious virus disease of cows and buffaloes. In Hindi it is often called khurpaka-munhpaka. Animals may get a high fever, blisters on the tongue, gums, feet and teats, drooling, lameness and a sudden drop in milk, and young calves can become very ill. The virus spreads very easily between animals and on people, clothes and vehicles. FMD is a notifiable disease, so owners should tell their veterinarian or the animal husbandry department about any suspected case.

The FMD virus is made of structural proteins, which form its outer shell, and non-structural proteins, or NSPs, which the virus makes only while it is multiplying inside an animal. Purified FMD vaccines contain mainly structural proteins, so vaccinated animals usually do not make antibodies to NSPs. This ELISA looks for antibodies to the 3ABC non-structural protein. Finding them suggests that the animal has been infected at some time, now or in the past, even if it has also been vaccinated. This is why the test is commonly used for herd surveillance and for checking animals before purchase or movement.

The test does not show which FMD type infected the animal, and it is not the first test for an animal with fresh blisters; for those, your vet may send blister fluid or tissue for tests that find the virus itself. Antibodies take time to appear, so very early infections can test negative. Now and then, an animal vaccinated many times can show these antibodies. A registered veterinarian reads the result with the herd's signs, vaccination history and other tests, and works with the animal husbandry department where needed.

How to prepare

No fasting is needed; blood is taken into a plain tube for serum, and tell the vet about the herd's FMD vaccination history and any recent blisters or lameness. The lab confirms the method, sample needs and reporting time when you book.

Reading the result

A registered veterinarian reads the result with the herd's signs, vaccination history and other tests. A positive result suggests past or current FMD infection, not just vaccination, though animals vaccinated many times can now and then react. A negative result does not rule out very early infection. Suspected FMD should be reported to your vet or the animal husbandry department.

Questions about FMD NSP (3ABC) Antibody ELISA

What does the FMD 3ABC NSP ELISA show?
It shows whether a cow or buffalo has antibodies to the non-structural proteins of FMD virus. These are made mainly after real infection, not vaccination, so a positive result suggests the animal has been infected at some time. Your vet reads it with the herd's vaccination history.
Can this test tell vaccinated animals from infected ones?
Largely, yes. Purified vaccines mainly cause antibodies to the virus's structural proteins, while this test looks for antibodies to non-structural proteins made during infection. Animals vaccinated many times can now and then react, so your vet reads the result with the vaccination history.
What should I do if I suspect FMD in my cows or buffaloes?
Call your veterinarian at once and tell the animal husbandry department, because FMD is notifiable and spreads very easily. Your vet will decide which samples and tests are needed. For animals with fresh blisters, tests that find the virus itself are usually used first.
Can I get FMD antibody testing in Bhopal?
Yes. Pashupatinath Vet Lab in Shahpura, Bhopal, collects samples free anywhere in Bhopal, and vets in other districts can send serum by courier or bus parcel. Call or WhatsApp +91 88175 01118 to book. Reports come on WhatsApp, email and in My Reports.

Before you book this test

Why this notice: so you know, before you book, how a test is done, what its limits are and who is responsible.

  • Where it is tested. Some tests are done wholly or partly by an independent partner laboratory. The lab tells you before booking if this test is sent out, and the report names the laboratory that did it.
  • What a result can and cannot tell you. No test is right every time. A normal result does not rule out every disease, and an abnormal result does not prove one. A registered veterinarian should read the report with the animal’s signs and history before any treatment is started, changed or stopped.
  • The sample. A sample that is clotted, too small, wrongly labelled, leaking or too old may be rejected or reported with a note, and the lab may ask for a fresh one.
  • Reporting time. Confirmed when you book. Times are estimates, not guarantees, and the lab confirms the time when you book.
  • Emergencies. A booking or a pending report must not delay urgent care. If an animal has collapsed, struggles to breathe, has fits or bleeds heavily, call a veterinarian at once.

Please read our Terms of Service, Medical Disclaimer, Privacy Policy and Refund and Cancellation Policy. Pashupatinath Vet Lab is operated by Duaji Healthcare and Tech Private Limited. This page is general information and does not replace a veterinarian’s advice.

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Foot-and-Mouth Disease: Sample and Notification Guide for Farmers

Foot-and-mouth disease (FMD) is a highly contagious notifiable disease that causes fever, blisters and a fall in milk. A vet takes the samples for RT-PCR and antibody tests, and owners should tell their veterinarian or the animal husbandry department.

18 Oct 2026

FMD NSP (3ABC) Antibody ELISA

Price on request

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Book online in two minutes. We collect the sample free of charge anywhere in Bhopal, or you can send it from any district of Madhya Pradesh.

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